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Single Domain Antibodies pp 399–416Cite as

Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 911))

Abstract

The successful medical application of single domain antibodies largely depends on their functionality. This feature is partly determined by the intrinsic stability of the single domain. Therefore a lot of research has gone into the elucidation of rules to uniformly increase stability of antibodies. Recently, a novel intra-domain disulfide bond was independently discovered by two research groups, after either rational design or careful investigation of the naturally occurring camelid antibody repertoire. By introducing this particular disulfide bond within a single domain antibody, the conformational stability can be increased in general. In this chapter it is described how to introduce this extra intra-domain disulfide bond and how to estimate the biophysical and biochemical impact of this cystine on the domain.

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Acknowledgements

No financial interest is declared by the authors. We would like to thank Ms. Kaede Lilian-Komaba, Ir. Jochen Govaert, and Dr. Ir. Cécile Vincke for critically reading the manuscript.

Author information

Authors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan

    Yoshihisa Hagihara

  2. Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium

    Dirk Saerens

  3. Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium

    Dirk Saerens

Authors
  1. Yoshihisa Hagihara
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  2. Dirk Saerens
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Corresponding author

Correspondence to Yoshihisa Hagihara .

Editor information

Editors and Affiliations

  1. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, 1050, Belgium

    Dirk Saerens

  2. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, 1050, Belgium

    Serge Muyldermans

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Hagihara, Y., Saerens, D. (2012). Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction. In: Saerens, D., Muyldermans, S. (eds) Single Domain Antibodies. Methods in Molecular Biology, vol 911. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-968-6_24

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  • DOI: https://doi.org/10.1007/978-1-61779-968-6_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-967-9

  • Online ISBN: 978-1-61779-968-6

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