Abstract
Efficient identification of antibodies, or any fragments thereof, displaying desired specificity and affinity is critical for the development of novel immunotherapeutics. Here we describe the adaptation of in vitro compartmentalization for the cell-free selection of Vκ and VH domain antibodies (dAbs™) from large combinatorial libraries. The dAbs™ are in vitro expressed in fusion to the N-terminus of single-chain variant of phage P22 Arc repressor DNA-binding domain that links the compartmentally expressed protein molecules to their encoding PCR fragment-based genes via cognate operator sites present on the DNA. Libraries of up to 1010 in size can be rapidly assembled and selected for improved affinity in equilibrium and off-rate conditions.
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Acknowledgements
The authors would like to thank Dr. Allart Stoop and Dr. Peter Ertl for helpful advice and suggestions. Dr. A. Sepp was an employee of Domantis Ltd. at the time this work was carried out.
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Sepp, A., Griffiths, A. (2012). Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization. In: Saerens, D., Muyldermans, S. (eds) Single Domain Antibodies. Methods in Molecular Biology, vol 911. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-968-6_12
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DOI: https://doi.org/10.1007/978-1-61779-968-6_12
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