Abstract
Here we describe postembedding immunoelectron microscopic technique applied to ultrathin sections of plant material. The method relies on the use of gold nanoparticles. The methods include tissue fixation, dehydration, embedding of plant specimens, and staining of semithin and ultrathin sections. The described method is suitable for localization of antigens including proteins (peroxisomal enzymes) and a low molecular substance such as ginsenoide-Rb1.
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Acknowledgements
The work was supported by the Nagasaki International University research fund and in part by a grant-in-aid (17570158) from the Ministry of Education, Science, Culture and Sport and by the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan.
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Yokota, S. (2012). Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_5
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DOI: https://doi.org/10.1007/978-1-61779-953-2_5
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Publisher Name: Humana Press, Totowa, NJ
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