Skip to main content
SpringerLink
Log in

Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery

  • Protocol
  • First Online:
Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

Abstract

Biomimetic nanoparticles are hybrid nanostructures in which the uppermost layer is similar to a cell membrane. This report provides instructions for the preparation and physical characterization of three different types of biomimetic nanoparticles: (1) polystyrene sulfate nanoparticles covered with one cationic dioctadecyldimethylammonium bilayer; (2) silica nanoparticles covered with one neutral phosphatidylcholine bilayer; (3) miconazole particles covered with one anionic dihexadecylphosphate (DHP) bilayer. These examples provide the basis for the preparation and characterization of novel nanoparticles from hydrophobic or hydrophilic and organic or inorganic nanoparticle cores covered with functional outer layers. The major concepts and technical details for obtaining the optimal lipid coverage of supporting cores and for nanoparticle characterization are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Carmona-Ribeiro AM, Midmore BR (1992) Synthetic bilayer adsorption onto polystyrene microspheres. Langmuir 8:801–806

    Article  CAS  Google Scholar 

  2. Carmona-Ribeiro AM (1992) Synthetic amphiphile vesicles. Chem Soc Rev 21:209–214

    Article  CAS  Google Scholar 

  3. Carmona-Ribeiro AM, Herrington TM (1993) Phospholipid adsorption onto polystyrene microspheres. J Colloid Interface Sci 156:19–24

    Article  CAS  Google Scholar 

  4. Rapuano R, Carmona-Ribeiro AM (1997) Physical adsorption of bilayer membranes on silica. J Colloid Interface Sci 193:104–109

    Article  PubMed  CAS  Google Scholar 

  5. Rapuano R, Carmona-Ribeiro AM (2000) Supported bilayers on silica. J Colloid Interface Sci 226:299–307

    Article  CAS  Google Scholar 

  6. Carmona-Ribeiro AM (2001) Bilayer vesicles and liposomes as interface agents. Chem Soc Rev 30:241–247

    Article  CAS  Google Scholar 

  7. Moura SP, Carmona-Ribeiro AM (2003) Cationic bilayer fragments on silica at low ionic strength: competitive adsorption and colloidal stability. Langmuir 19:6664–6667

    Article  CAS  Google Scholar 

  8. Carmona-Ribeiro AM (2003) Bilayer-forming synthetic lipids: drugs or carriers? Curr Med Chem 10:2425–2446

    Article  PubMed  CAS  Google Scholar 

  9. Pacheco LF, Carmona-Ribeiro AM (2003) Effects of synthetic lipids on solubilization and colloid stability of hydrophobic drugs. J Colloid Interface Sci 258:146–154

    Article  PubMed  CAS  Google Scholar 

  10. Pereira EMA, Vieira DB, Carmona-Ribeiro AM (2004) Cationic bilayers on polymeric particles: effect of low NaCl concentration on surface coverage. J Phys Chem B 108:11490–11495

    Article  CAS  Google Scholar 

  11. Moura SP, Carmona-Ribeiro AM (2005) Biomimetic particles: optimization of phospholipid bilayer coverage on silica and colloid stabilization. Langmuir 21:10160–10164

    Article  PubMed  CAS  Google Scholar 

  12. Moura SP, Carmona-Ribeiro AM (2006) Biomimetic particles for isolation and reconstitution of receptor function. Cell Biochem Biophys 44:446–452

    Article  PubMed  CAS  Google Scholar 

  13. Lincopan N, Carmona-Ribeiro AM (2006) Lipid-covered drug particles: combined action of dioctadecyldimethylammonium bromide and amphotericin B or miconazole. J Antimicrob Chemother 58:66–75

    Article  PubMed  CAS  Google Scholar 

  14. Lincopan N, Espíndola NM, Vaz AJ et al (2007) Cationic supported lipid bilayers for antigen presentation. Int J Pharm 340:216–222

    Article  PubMed  CAS  Google Scholar 

  15. Lincopan N, Santana MRA, Faquim-Mauro E et al. (2009) Silica-based cationic bilayers as immunoadjuvants. BMC Biotechnol 9:5. http://www.biomedcentral.com/1472-6750/9/5

  16. Lincopan N, Espíndola NM, Vaz AJ et al (2009) Novel immunoadjuvants based on cationic lipid: preparation, characterization and activity in vivo. Vaccine 27:5760–5771

    Article  PubMed  CAS  Google Scholar 

  17. Moura SP, Carmona-Ribeiro AM (2007) Adsorption behavior of DODAB/DPPC vesicles on silica. J Colloid Interface Sci 313:519–526

    Article  PubMed  CAS  Google Scholar 

  18. Grabowski E, Morrison I (1983) Particle size distribution from analysis of quasi-elastic light scattering data. In: Dahneke B (ed) Measurements of suspended particles by quasi-elastic light scattering. Wiley-Interscience, New York, pp 199–236

    Google Scholar 

  19. Vieira DB, Carmona-Ribeiro AM (2008) Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro. J Nanobiotechnology 6:6. http://www.jnanobiotechnology.com/content/6/1/6

    Google Scholar 

  20. Nascimento DB, Rapuano R, Sesso A et al (1998) Counterion effects on properties of cationic vesicles. Langmuir 14:7387–7391

    Article  CAS  Google Scholar 

  21. Carmona-Ribeiro AM, Castuma CE, Sesso A et al (1991) Bilayer structure and stability in dihexadecyl phosphate dispersions. J Phys Chem US 95:5361–5366

    Article  CAS  Google Scholar 

  22. Leão-Silva AC, Naves AF, Pereira EMA et al (2011) Assembly of horse radish peroxidase within supported cationic bilayers. Biotechnol Prog 27(5):1433–1441

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by FAPESP and CNPq. Professor Frank Quina is gratefully acknowledged for reviewing the manuscript.

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Biocolloids Lab, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil

    Ana Maria Carmona-Ribeiro

Authors
  1. Ana Maria Carmona-Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ana Maria Carmona-Ribeiro .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Carmona-Ribeiro, A.M. (2012). Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_22

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-953-2_22

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation