Abstract
Ordered collections of barcoded deletion mutants can be screened rapidly in mixed cultures to uncover resistance and sensitivity phenotypes associated with the loss of a gene. As such, they are invaluable tools for assigning gene function in the post-genomic era. In this protocol, we describe methodologies for creating and employing barcoded deletion mutants in competitive screens as well as how to analyze the ensuing results.
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Acknowledgements
This research was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development (E.C.H. and G.S.) and by the Pharmacology Research Associate Program of the National Institute of General Medical Sciences (E.C.H.).
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Hobbs, E.C., Storz, G. (2012). Competition Assays Using Barcoded Deletion Strains to Gain Insight into Small RNA Function. In: Keiler, K. (eds) Bacterial Regulatory RNA. Methods in Molecular Biology, vol 905. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-949-5_5
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DOI: https://doi.org/10.1007/978-1-61779-949-5_5
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-949-5
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