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Activity of Small RNAs on the Stability of Targeted mRNAs In Vivo

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Bacterial Regulatory RNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 905))

Abstract

Northern blots are extremely useful to monitor the steady state level of small regulatory RNAs (sRNAs) as well as their target mRNAs. In combination with the drug rifampicin, which blocks cellular transcription, Northern blots can be used to determine the stability of sRNAs and mRNAs. Here we describe a protocol to assess the activity of the sRNA RyhB on the stability of targeted mRNAs sodB, fumA, and iscRSUA. We also describe how to identify a sRNA-induced initial cleavage site on a target mRNA. This protocol can be used for any sRNAs and their target mRNAs.

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Acknowledgments

We thank Gilles Dupuis for comments on the manuscript. This work was funded by an operating grant MOP69005 to E.M. from the Canadian Institutes for Health Research (CIHR). G.D. is a Ph.D. Scholar from the FQRNT (Fonds Québécois de la Recherche sur la Nature et les Technologies). E.M. is a FRSQ (Fonds de la Recherche en Santé du Québec) Junior II Scholar.

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Authors and Affiliations

  1. Department of Biochemistry, University of Sherbrooke, Sherbrooke, QC, Canada

    Guillaume Desnoyers & Eric Massé

Authors
  1. Guillaume Desnoyers
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  2. Eric Massé
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Corresponding author

Correspondence to Eric Massé .

Editor information

Editors and Affiliations

  1. , Dept. of Biochemistry and Molecular Biol, The Pennsylvania State University, Althouse Laboratory 0401, University Park, 16802, USA

    Kenneth C. Keiler

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Desnoyers, G., Massé, E. (2012). Activity of Small RNAs on the Stability of Targeted mRNAs In Vivo. In: Keiler, K. (eds) Bacterial Regulatory RNA. Methods in Molecular Biology, vol 905. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-949-5_16

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  • DOI: https://doi.org/10.1007/978-1-61779-949-5_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-948-8

  • Online ISBN: 978-1-61779-949-5

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