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Quantifying Hematopoietic Stem and Progenitor Cell Mobilization

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Stem Cell Mobilization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 904))

Abstract

Allogeneic donor blood cells and autologous peripheral blood leukocytes (PBL), obtained following ­clinical mobilization procedures, are routinely used as a major source of hematopoietic stem and progenitor cells (HSPC) for transplantation protocols. It is, therefore, essential to evaluate and to quantify the extent by which the HSPC are mobilized and enriched in the circulation in correlation with their long-term hematopoietic reconstitution capacity. In this chapter, we describe quantitative methods that measure the number of mobilized HSPC according to specific criteria, as well as their functional properties in vitro and in vivo. The described assays are useful for assessment of progenitor cell mobilization as applied to both human and murine HSPC.

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Authors and Affiliations

  1. Department of Immunology, Weizmann Institute of Science, Rehovot, Israel

    Shiri Gur-Cohen, Kfir Lapid & Tsvee Lapidot

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  1. Shiri Gur-Cohen
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  2. Kfir Lapid
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  3. Tsvee Lapidot
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Correspondence to Tsvee Lapidot .

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Editors and Affiliations

  1. Science Center at Houston, Inst. Molecular Medicine, University of Texas, Pressler Street 1825, Houston, 77030, Texas, USA

    Mikhail G. Kolonin

  2. Health Science Center, Houston, Inst. Molecular Medicine, University of Texas, Pressler Street 1825, Houston, 77030, Texas, USA

    Paul J. Simmons

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Gur-Cohen, S., Lapid, K., Lapidot, T. (2012). Quantifying Hematopoietic Stem and Progenitor Cell Mobilization. In: Kolonin, M., Simmons, P. (eds) Stem Cell Mobilization. Methods in Molecular Biology, vol 904. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-943-3_2

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  • DOI: https://doi.org/10.1007/978-1-61779-943-3_2

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