Abstract
Real-time PCR has engendered wide acceptance for quantitation of hepatitis B virus (HBV) DNA in the blood due to its improved rapidity, sensitivity, reproducibility, and reduced contamination. Here we describe a cost-effective and highly sensitive HBV real-time quantitative assay based on the light upon extension real-time PCR platform and a simple and reliable HBV DNA preparation method using silica-coated magnetic beads.
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Acknowledgements
The work was funded by the international cooperation projects of Jilin Province Science and Technology Agency (20080728). The authors would like to thank the kind support from all colleagues of the laboratory.
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© 2012 Springer Science+Business Media New York
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Li, G., Li, W., Liu, L. (2012). Protocol for the Use of Light Upon Extension Real-Time PCR for the Determination of Viral Load in HBV Infection. In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_18
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DOI: https://doi.org/10.1007/978-1-61779-937-2_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-936-5
Online ISBN: 978-1-61779-937-2
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