Abstract
Quantitative measurements of serum hepatitis B virus (HBV) DNA are useful for tailoring of treatment schedules and the monitoring of HBV replication during therapy. We developed a novel fluorescence-based quantitative real-time PCR for quantitating HBV DNA based on the duplex mutation primers principle, in which signal is generated by melting a duplex mutation primer during renaturation. The duplex mutation primers are much more specific than double-stranded DNA dyes like SYBR Green I and, unlike other probes, do not require the double-labeled synthesis of fluorophore and quencher on the same molecule.
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Acknowledgments
This work was supported by grant HjKj200524 and Hj2010-20 from the Department of Education of Hainan province.
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© 2012 Springer Science+Business Media New York
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Xia, QF. (2012). Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV. In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_16
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DOI: https://doi.org/10.1007/978-1-61779-937-2_16
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-937-2
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