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Diagnosis of Sexually Transmitted Diseases pp 169–175Cite as

Extraction of DNA from Dried Blood in the Diagnosis of Congenital CMV Infection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 903))

Abstract

Viral DNA detection in dried blood spotted on filter paper, dried blood spots (DBS), is valuable in the diagnosis of viral infections, with at the moment congenital cytomegalovirus (CMV) being the most common application. CMV detection in clinical samples taken within the first 2–3 weeks after birth differentiates congenital CMV infection from the in general harmless postnatal acquired cytomegalovirus infection. DBS render the possibility to diagnose congenital CMV infection retrospectively, e.g., when late-onset hearing loss, the most frequently encountered symptom of congenital CMV infection, becomes manifest. Additionally, CMV DNA detection in DBS can be of usage in recently advocated newborn screening on congenital CMV infection. The procedure of CMV DNA detection in DBS consists of two separate steps: (1) DNA extraction from the DBS, followed by (2) CMV DNA amplification. Here, we describe two efficient methods for the extraction of DNA from DBS. Sensitivity, specificity, and applicability of the methods for high-throughput usage are discussed.

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Clinical Microbiological Laboratory, Leiden University Medical Center (LUMC), Leiden, The Netherlands

    Jutte J. C. de Vries

  2. Department of Medical Microbiology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

    Maria Barbi, Sandro Binda & Eric C. J. Claas

Authors
  1. Jutte J. C. de Vries
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  2. Maria Barbi
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  3. Sandro Binda
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  4. Eric C. J. Claas
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Corresponding author

Correspondence to Jutte J. C. de Vries .

Editor information

Editors and Affiliations

  1. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Colin R. MacKenzie

  2. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Birgit Henrich

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de Vries, J.J.C., Barbi, M., Binda, S., Claas, E.C.J. (2012). Extraction of DNA from Dried Blood in the Diagnosis of Congenital CMV Infection. In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_10

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  • DOI: https://doi.org/10.1007/978-1-61779-937-2_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-936-5

  • Online ISBN: 978-1-61779-937-2

  • eBook Packages: Springer Protocols

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