Abstract
Because of their prevalence in drugged driving and other medicolegal investigations, cannabinoids are routinely analyzed by forensic laboratories. Until relatively recently, these analyses were performed by gas chromatography coupled to mass spectrometry (GC-MS). However, the need for derivatization and extensive sample preparation made GC-MS approaches tedious and time consuming. As a consequence, many laboratories have explored alternative analysis techniques. The advent of more affordable liquid chromatography-mass spectrometry (LC-MS) instruments and the utility of atmospheric pressure ionization sources have made LC-MS a promising alternative to GC-MS for the detection and quantitation of cannabinoids in forensic applications.
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References
Weinmann W, Goerner M, Vogt S, Goerke R, Pollak S (2001) Fast confirmation of 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH) in urine by LC/MS/MS using negative atmospheric-pressure chemical ionisation (APCI). Forensic Sci Int 121(1–2):103–107
Skopp G, Pötsch L (2002) Stability of 11-nor-delta(9)-carboxy-tetrahydrocannabinol glucuronide in plasma and urine assessed by liquid chromatography-tandem mass spectrometry. Clin Chem 48(2):301–306
Valiveti S, Stinchcomb AL (2004) Liquid chromatographic-mass spectrometric quantitation of Delta9-tetrahydrocannabinol and two metabolites in pharmacokinetic study plasma samples. J Chromatogr B: Analyt Technol Biomed Life Sci 803(2):243–248
Maralikova B, Weinmann W (2004) Simultaneous determination of Delta9-tetrahydrocannabinol, 11-hydroxy-Delta9-tetrahydrocannabinol and 11-nor-9-carboxy-Delta9-tetrahydrocannabinol in human plasma by high-performance liquid chromatography/tandem mass spectrometry. J Mass Spectrom 39(5):526–531
Concheiro M, de Castro A, Quintela O, Cruz A, López-Rivadulla M (2004) Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry. J Chromatogr B: Analyt Technol Biomed Life Sci 810(2):319–324
Valiveti S, Hammell DC, Earles DC, Stinchcomb AL (2005) LC-MS method for the estimation of delta8-THC and 11-nor-delta8-THC-9-COOH in plasma. J Pharm Biomed Anal 38(1):112–118
Laloup M, Ramirez Fernandez Mdel M, Wood M, De Boeck G, Henquet C, Maes V, Samyn N (2005) Quantitative analysis of delta9-tetrahydrocannabinol in preserved oral fluid by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1082(1):15–24
Grauwiler SB, Scholer A, Drewe J (2007) Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts. J Chromatogr B: Analyt Technol Biomed Life Sci 850(1–2):515–522
Quintela O, Andrenyak DM, Hoggan AM, Crouch DJ (2007) A validated method for the detection of Delta 9-tetrahydrocannabinol and 11-nor-9-carboxy-Delta 9-tetrahydrocannabinol in oral fluid samples by liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. J Anal Toxicol 31(3):157–164
Teixeira H, Verstraete A, Proença P, Corte-Real F, Monsanto P, Vieira DN (2007) Validated method for the simultaneous determination of Delta9-THC and Delta9-THC-COOH in oral fluid, urine and whole blood using solid-phase extraction and liquid chromatography-mass spectrometry with electrospray ionization. Forensic Sci Int 170(2–3):148–155
Jamey C, Szwarc E, Tracqui A, Ludes B (2008) Determination of cannabinoids in whole blood by UPLC-MS-MS. J Anal Toxicol 32(5):349–354
Stephanson N, Josefsson M, Kronstrand R, Beck O (2008) Accurate identification and quantification of 11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid in urine drug testing: evaluation of a direct high efficiency liquid chromatographic-mass spectrometric method. J Chromatogr B: Analyt Technol Biomed Life Sci 871(1):101–108
Del Mar Ramirez Fernandez M, De Boeck G, Wood M, Lopez-Rivadulla M, Samyn N (2008) Simultaneous analysis of THC and its metabolites in blood using liquid chromatography-tandem mass spectrometry. J Chromatogr B: Analyt Technol Biomed Life Sci 875(2):465–470
Coulter C, Miller E, Crompton K, Moore C (2008) Tetrahydrocannabinol and two of its metabolites in whole blood using liquid chromatography-tandem mass spectrometry. J Anal Toxicol 32(8):653–658
Coulter C, Taruc M, Tuyay J, Moore C (2009) Quantitation of tetrahydrocannabinol in hair using immunoassay and liquid chromatography with tandem mass spectrometric detection. Drug Test Anal 1(5):234–239
Jagerdeo E, Schaff JE, Montgomery MA, LeBeau MA (2009) A semi-automated solid-phase extraction liquid chromatography/tandem mass spectrometry method for the analysis of tetrahydrocannabinol and metabolites in whole blood. Rapid Commun Mass Spectrom 23(17):2697–2670
Fernández Mdel M, Wille SM, Samyn N, Wood M, López-Rivadulla M, De Boeck G (2009) On-line solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for high throughput analysis of 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid in urine. J Chromatogr B: Analyt Technol Biomed Life Sci 877(22):2153–2157
Robandt PV, Klette KL, Sibum M (2009) Automated solid-phase extraction-liquid chromatography-tandem mass spectrometry analysis of 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid in human urine specimens: application to a high-throughput urine analysis laboratory. J Anal Toxicol 33(8):456–460
Elian AA, Hackett J (2009) Solid-phase extraction and analysis of THC and carboxy-THC from whole blood using a novel fluorinated solid-phase extraction sorbent and fast liquid chromatography-tandem mass spectrometry. J Anal Toxicol 33(8):461–468
Chebbah C, Pozo OJ, Deventer K, Van Eenoo P, Delbeke FT (2010) Direct quantification of 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in urine by liquid chromatography/tandem mass spectrometry in relation to doping control analysis. Rapid Commun Mass Spectrom 24:1133–1141
Jagerdeo E, Montgomery MA, Karas RP, Sibum M (2010) A fast method for screening and/or quantitation of tetrahydrocannabinol and metabolites in urine by automated SPE/LC/MS/MS. Anal Bioanal Chem 398(1):329–338
Van Hout MWJ, Niederländer HAG et al (2003) New developments in integrated sample preparation for bioanalysis. In: Wilson ID (ed) Bioanalytical separations. Elsevier, Amsterdam
Stolker AA, van Schoonhoven J, de Vries AJ, Bobeldijk-Pastorova I, Vaes WH, van den Berg R (2004) Determination of cannabinoids in cannabis products using liquid chromatography-ion trap mass spectrometry. J Chromatogr A 1058(1–2):143–151
Foltz RL, McGinnis KM, Chinn DM (1983) Quantitative measurement of delta 9-tetrahydrocannabinol and two major metabolites in physiological specimens using capillary column gas chromatography negative ion chemical ionization mass spectrometry. Biomed Mass Spectrom 10(5):316–323
Annesley TM (2003) Ion suppression in mass spectrometry. Clin Chem 49(7):1041–1044
Kronstrand R, Josefsson M (2006) Quantication using LC-MS. In: Polettini A (ed) Applications of LC-MS in toxicology. Pharmaceutical, London
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Quintela, O., Crouch, D.J. (2012). The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 902. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-934-1_7
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DOI: https://doi.org/10.1007/978-1-61779-934-1_7
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