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LC-MS in Drug Analysis pp 167–173Cite as

Simultaneous Determination of Cyclosporine, Sirolimus, and Tacrolimus in Whole Blood Using Liquid Chromatography–Tandem Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 902))

Abstract

A multiple reaction monitoring, positive ionization electrospray, liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is described for the simultaneous quantification of cyclosporine, sirolimus, and tacrolimus in human whole blood. Proteins in the samples are precipitated with a mixture of methanol and zinc sulfate. The supernatant is injected into the LC-MS/MS for analysis. Chromatography involves the use of a C18 column and ammonium acetate/water/methanol-containing mobile phases. The MS/MS is operated in positive ion electrospray mode. Quantification is achieved by comparing peak area ratios of MRMs of analytes and internal standards with that of calibrators. Calibration curves are constructed from peak area ratios of MRMs of calibrators and internal standards versus concentrations. MRMs used are ascomycin (m/z 809.5 → 756.5), cyclosporine A (m/z 1,219.9 → 1,203.1), cyclosporine D (m/z 1,234.0 → 1,217.0), sirolimus (m/z 931.6 → 864.5), and tacrolimus (m/z 821.5 → 768.4).

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Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of MO Kansas City School of Medicine, Children’s Mercy Hospitals and Clinics, Kansas City, MO, USA

    Uttam Garg & Ada Munar

  2. Department of Pathology and Laboratory Medicine, University of MO kansas City School of Medicine, Children’s Mercy Hospitals and Clinics, Kansas City, MO, USA

    C. Clinton Frazee III

Authors
  1. Uttam Garg
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  2. Ada Munar
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  3. C. Clinton Frazee III
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Corresponding author

Correspondence to Uttam Garg .

Editor information

Editors and Affiliations

  1. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Loralie J. Langman

  2. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Christine L. H. Snozek

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© 2012 Springer Science+Business Media, LLC

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Garg, U., Munar, A., Frazee, C.C. (2012). Simultaneous Determination of Cyclosporine, Sirolimus, and Tacrolimus in Whole Blood Using Liquid Chromatography–Tandem Mass Spectrometry. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 902. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-934-1_15

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  • DOI: https://doi.org/10.1007/978-1-61779-934-1_15

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-933-4

  • Online ISBN: 978-1-61779-934-1

  • eBook Packages: Springer Protocols

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