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Intrinsically Disordered Protein Analysis pp 67–83Cite as

In-Cell NMR in Mammalian Cells: Part 3

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 895))

Abstract

Irrespective of how isotope-labeled proteins are delivered into mammalian cells, laboratory routines are needed to assess the quality of the resulting in-cell NMR samples. These include methods to evaluate overall cell viability, protein transduction efficiency, intracellular protein concentration, localization, and stability. In addition, quality control experiments to assess protein leakage from manipulated cells are of particular importance for in-cell NMR experiments. The purpose of this chapter is to outline qualitative and quantitative methods to determine general biological properties of in-cell NMR samples in order to ensure the highest possible standards for in-cell NMR studies.

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Author information

Authors and Affiliations

  1. Department of NMR-assisted Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP Berlin), Berlin, Germany

    Beata Bekei, Honor May Rose, Michaela Herzig & Philipp Selenko

  2. Department of Chemical Biology and Department of NMR-assisted Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP Berlin), Berlin, Germany

    Heike Stephanowitz & Eberhard Krause

Authors
  1. Beata Bekei
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  2. Honor May Rose
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  3. Michaela Herzig
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  4. Heike Stephanowitz
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  5. Eberhard Krause
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  6. Philipp Selenko
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Corresponding author

Correspondence to Philipp Selenko .

Editor information

Editors and Affiliations

  1. Department of Molecular Medicine, College of Medicine, University of South Florida, Bruce B. Downs Blvd 12901, Tampa, 33612, Florida, USA

    Vladimir N. Uversky

  2. Dept. Biochemistry & Molecular Biology, Center for Computational Biology &, Indiana University, West 10th Street 410, Indianapolis, 46202, Indiana, USA

    A. Keith Dunker

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Bekei, B., Rose, H.M., Herzig, M., Stephanowitz, H., Krause, E., Selenko, P. (2012). In-Cell NMR in Mammalian Cells: Part 3. In: Uversky, V., Dunker, A. (eds) Intrinsically Disordered Protein Analysis. Methods in Molecular Biology, vol 895. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-927-3_6

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  • DOI: https://doi.org/10.1007/978-1-61779-927-3_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-926-6

  • Online ISBN: 978-1-61779-927-3

  • eBook Packages: Springer Protocols

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