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Wide-Line NMR and Protein Hydration

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Intrinsically Disordered Protein Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 895))

Abstract

In this chapter, the reader is introduced to the basics of wide-line NMR, with particular focus on the following: (1) basic theoretical and experimental NMR elements, necessary before switching the spectrometer and designing the experiment, (2) models/theories for the interpretation of measured data, (3) definition of wide-line NMR spectrometry, the description of the measurement and evaluation variants, useful hints for the novice, (4) advice on selecting the solvent, which is not a trivial task, (5) a note of warning that not all data are acceptable in spite of the statistical confidence. Finally, we wrap up the chapter with the results on two proteins (a globular and an intrinsically disordered).

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Acknowledgments

This work was supported by the Hungarian Academy of Sciences, the International Senior Research Fellowship ISRF 067595 from the Wellcome Trust, and a Korean–Hungarian Joint Laboratory grant from Korea Research Council of Fundamental Science and Technology (KRCF).

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Authors and Affiliations

  1. Research Institute for Solid State Physics and Optics, Hungarian Academy of Sciences, Budapest, Hungary

    K. Tompa & M. Bokor

  2. Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary

    P. Tompa

Authors
  1. K. Tompa
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  2. M. Bokor
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  3. P. Tompa
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Corresponding author

Correspondence to M. Bokor .

Editor information

Editors and Affiliations

  1. Department of Molecular Medicine, College of Medicine, University of South Florida, Bruce B. Downs Blvd 12901, Tampa, 33612, Florida, USA

    Vladimir N. Uversky

  2. Dept. Biochemistry & Molecular Biology, Center for Computational Biology &, Indiana University, West 10th Street 410, Indianapolis, 46202, Indiana, USA

    A. Keith Dunker

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Tompa, K., Bokor, M., Tompa, P. (2012). Wide-Line NMR and Protein Hydration. In: Uversky, V., Dunker, A. (eds) Intrinsically Disordered Protein Analysis. Methods in Molecular Biology, vol 895. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-927-3_13

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  • DOI: https://doi.org/10.1007/978-1-61779-927-3_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-926-6

  • Online ISBN: 978-1-61779-927-3

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