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Microbial Carotenoids From Fungi pp 251–262Cite as

DNA Assembler Method for Construction of Zeaxanthin-Producing Strains of Saccharomyces cerevisiae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 898))

Abstract

DNA assembler enables design and rapid construction of biochemical pathways in a one-step fashion by exploitation of the in vivo homologous recombination mechanism in Saccharomyces cerevisiae. It has many applications in pathway engineering, metabolic engineering, combinatorial biology, and synthetic biology. Here we use the zeaxanthin biosynthetic pathway as an example to describe the key steps in the construction of pathways containing multiple genes using the DNA assembler approach. Methods for the construction of the clones, S. cerevisiae transformation, and zeaxanthin production and detection are shown.

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Author information

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Zengyi Shao & Huimin Zhao

  2. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Yunzi Luo

  3. Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Huimin Zhao

Authors
  1. Zengyi Shao
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  2. Yunzi Luo
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  3. Huimin Zhao
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Corresponding author

Correspondence to Huimin Zhao .

Editor information

Editors and Affiliations

  1. Parque Tecnológico de León, Gadea Biopharma, Boecillo 47151, Valladolid, 24009, León, Spain

    José-Luis Barredo

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Shao, Z., Luo, Y., Zhao, H. (2012). DNA Assembler Method for Construction of Zeaxanthin-Producing Strains of Saccharomyces cerevisiae . In: Barredo, JL. (eds) Microbial Carotenoids From Fungi. Methods in Molecular Biology, vol 898. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-918-1_17

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  • DOI: https://doi.org/10.1007/978-1-61779-918-1_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-917-4

  • Online ISBN: 978-1-61779-918-1

  • eBook Packages: Springer Protocols

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