Abstract
The yeasts Xanthophyllomyces dendrorhous (teleomorph) and Phaffia rhodozyma (anamorph) are of basidiomycetous affinity and have the unique property among yeasts of producing the carotenoid pigment astaxanthin. Astaxanthin imparts the attractive coloration to salmonids, crustaceans, and several birds such as the flamingo, and it has considerable economic value. Microbiological and genetic techniques for manipulation are rudimentary in the yeast, while their utility would be valuable for strain development including hypermutants that overproduce astaxanthin. Here we describe methods for manipulation of the yeast, including induction of the sexual stage with basidiospore formation, methods for isolation of mutants (particularly mutants affected in carotenoid biosynthesis) as well as techniques for isolation and analysis of carotenoids. These methods are valuable for understanding the biology and enhancing the biotechnology value of the yeast.
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References
Phaff HJ, Miller MW, Yoneyama M, Soneda M (1972) A comparative study of the yeast florae associated with trees on the Japanese islands and on the west coast of North America. In: Terui G (ed) Fermentation technology today: proceedings of fourth international fermentation symposium. Society of Fermentation Technology, Osaka, pp 759–774
Miller MW, Yoneyama M, Soneda M (1976) Phaffia, a new yeast species in the in the Deuteromycotina (Blastomycetes). Int J Syst Bacteriol 26:88–91
Fell JW, Johnson EA (2011) Phaffia M. W. Miller, Yoneyama & Soneda (1976). In: Kurtzman CP, Fell JW, Boekhout T (ed) The yeasts. A taxonomic study, 5th edn. Elsevier, Amsterdam, p. 1853–1855
Fell JW, Johnson EA, Scorzetti G (2011) Xanthophyllomyces Golubev (1995). In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts. A taxonomic study, 5th edn. Elsevier, Amsterdam, pp 1555–1595
Andrewes AG, Phaff HJ, Starr MP (1976) Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting yeast. Phytochemistry 15:1003–1007
Johnson EA (2003) Phaffia rhodozyma: a colorful odyssey. Int Microbiol 6:169–174
Johnson EA, Schroeder WA (1995) Microbial carotenoids. Adv Biochem Eng Biotechnol 53:119–178
Golubev WI (1995) Perfect state of Rhodomyces dendrorhous. Yeast 11:101–110
Retamales P, Hermosilla G, Leon R, MartÃnez C, Jimenez A, Cifuentes V (2002) Development of the sexual reproductive cycle of Xanthophyllomyces dendrorhous. J Microbiol Methods 48:87–93
An G-H, Schuman DB, Johnson EA (1989) Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl Environ Microbiol 55:116–124
An GH, Bielich J, Auerbach R, Johnson EA (1991) Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting. Biotechnology (NY) 9:70–73
Echavarri-Erasun C, Johnson E (2002) Fungal carotenoids. In: Khachatourians G, Arora D (eds) Applied mycology and biotechnology. Elsevier Science, Amsterdam, pp 45–85
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Lin, G., Bultman, J., Johnson, E.A., Fell, J.W. (2012). Genetic Manipulation of Xanthophyllomyces dendrorhous and Phaffia rhodozyma . In: Barredo, JL. (eds) Microbial Carotenoids From Fungi. Methods in Molecular Biology, vol 898. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-918-1_16
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DOI: https://doi.org/10.1007/978-1-61779-918-1_16
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