Abstract
An IISA-ATP (integrated in situ analyzer for ATP) was developed for the quantitative determination of ATP (adenosine-5′-triphosphate) in ocean environments, including the deep-sea. Total (intracellular and dissolved) ATP was quantitatively determined using a simple luciferin-luciferase (L-L) bioluminescence assay. A microfluidic device was incorporated as a core functional element in the IISA-ATP for performing cell lysis and carrying out the L-L reaction. The IISA-ATP has the capability to process environmental samples in the laboratory and in situ. Here, we provide an overview of the performance of the microfluidic device and the IISA-ATP on the basis of results of an in situ trial.
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Acknowledgements
The authors are grateful to Dr. H. Yamamoto (Japan Agency for Marine-Earth and Technology: JAMSTEC) and Dr. Y. Furushima (JAMSTEC) for their assistance during the in situ trial of the IISA-ATP in Okinawa. This study was supported by a grant-in-aid by the Scientific Research (S) and Development of Fundamental Tools Program for underwater resource survey from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT).
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Fukuba, T., Fujii, T. (2012). Bioluminescence Detection for ATP Quantification Using Microfluidic Device. In: Tiquia-Arashiro, S. (eds) Molecular Biological Technologies for Ocean Sensing. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-915-0_10
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DOI: https://doi.org/10.1007/978-1-61779-915-0_10
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