Abstract
Monoclonal antibodies to specific vaccinia virus (VACV) proteins are valuable reagents in studies of VACV. In this chapter, we describe methods of generating a panel of monoclonal antibodies that recognize a variety of VACV proteins in their native conformation in infected cells. The antibodies thus generated recognize mostly VACV proteins that are involved in virion assembly or/and are major antigens in smallpox vaccine. These antibodies are useful for tracking distinct steps in virion assembly and for studying the B cell epitopes in smallpox vaccine.
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Acknowledgments
Y.X is supported by NIH grant AI079217 and contract 272200900048C-0-0-1.
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Meng, X., Xiang, Y. (2012). Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus. In: Isaacs, S. (eds) Vaccinia Virus and Poxvirology. Methods in Molecular Biology, vol 890. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-876-4_13
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DOI: https://doi.org/10.1007/978-1-61779-876-4_13
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