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Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production

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Prenatal Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 891))

Abstract

Gene therapy vectors based on viruses are the most effective gene delivery systems in use today and although efficient at gene transfer their potential toxicity (Hacein-Bey-Abina et al., Science 302:415–419, 2003) provides impetus for the development of safer non-viral alternatives. An ideal vector for human gene therapy should deliver sustainable therapeutic levels of gene expression without affecting the viability of the host at either the cellular or somatic level. Vectors, which comprise entirely human elements, may provide the most suitable method of achieving this. Non-viral vectors are attractive alternatives to viral gene delivery systems because of their low toxicity, relatively easy production, and great versatility. The development of more efficient, economically prepared, and safer gene delivery vectors is a crucial prerequisite for their successful clinical application and remains a primary strategic task of gene therapy research.

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Authors and Affiliations

  1. Faculty of Medicine, Molecular and Cellular Medicine Section, National Heart and Lung Institute, Imperial College London, London, UK

    Suet Ping Wong PhD, Orestis Argyros PhD & Richard P. Harbottle PhD

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  1. Suet Ping Wong PhD
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  2. Orestis Argyros PhD
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  3. Richard P. Harbottle PhD
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Correspondence to Richard P. Harbottle PhD .

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Editors and Affiliations

  1. National Heart & Lung Inst., Molecular & Cellular Medicine Sec., Imperial College London, Imperial College Road, London, SW7 2AZ, United Kingdom

    Charles Coutelle

  2. Reader in Gene Transfer Technology, Gene Transfer Technology Group, University College London, Chenies Mews 86-96, London, WC1E 6HX, United Kingdom

    Simon N. Waddington

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Wong, S.P., Argyros, O., Harbottle, R.P. (2012). Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production. In: Coutelle, C., Waddington, S. (eds) Prenatal Gene Therapy. Methods in Molecular Biology, vol 891. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-873-3_7

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  • DOI: https://doi.org/10.1007/978-1-61779-873-3_7

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