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DNA Methylation Screening and Analysis

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Book cover Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 889))

Abstract

DNA methylation is an epigenetic form of gene regulation that is universally important throughout the life course, especially during in utero and postnatal development. DNA methylation aids in cell cycle regulation and cellular differentiation processes. Previous studies have demonstrated that DNA methylation profiles may be altered by diet and the environment, and that these profiles are especially vulnerable during development. Thus, it is important to understand the role of DNA methylation in developmental governance and subsequent disease progression. A variety of molecular methods exist to assay for global, gene-specific, and epigenome-wide methylation. Here we describe these methods and discuss their relative strengths and limitations.

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Acknowledgments

This work was supported by NIH grant ES017524. Support for KES and MSN was provided by an Institutional Training Grant from the National Institute of Environmental Health Sciences (NIEHS), NIH (T32 ES007062).

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Authors and Affiliations

  1. Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, USA

    Karilyn E. Sant, Muna S. Nahar & Dana C. Dolinoy Ph.D.

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  1. Karilyn E. Sant
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  2. Muna S. Nahar
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  3. Dana C. Dolinoy Ph.D.
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Correspondence to Dana C. Dolinoy Ph.D. .

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Editors and Affiliations

  1. Toxicology Program, Dept. Environmental Health Sciences, University of Michigan, Washington Heights 1420, Ann Arbor, 48109-2029, Michigan, USA

    Craig Harris

  2. , Department of Pediatrics, Emory University, Uppergate Drive NE., 2015Suite 350, Atlanta, 30322, Georgia, USA

    Jason M. Hansen

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Sant, K.E., Nahar, M.S., Dolinoy, D.C. (2012). DNA Methylation Screening and Analysis. In: Harris, C., Hansen, J. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 889. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-867-2_24

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  • DOI: https://doi.org/10.1007/978-1-61779-867-2_24

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