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Electron Microscopy

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Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 887))

Abstract

Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural details of individual cells and, on the other hand, are large enough to allow a correlation with light micrographs. The second protocol describes a technique for estimating mineral densities of hard tissues using backscattered electron images obtained with a scanning electron microscope. This technique can be used to analyze the mineralization processes which occur throughout tooth formation.

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Acknowledgments

The authors thank Jacqueline Hofmann-Lobsiger and Steven Reese for the skilful preparation of the specimens used for the illustrations of this work.

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Authors and Affiliations

  1. Department of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland

    Hans U. Luder & Margrit Amstad-Jossi

Authors
  1. Hans U. Luder
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  2. Margrit Amstad-Jossi
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Corresponding author

Correspondence to Hans U. Luder .

Editor information

Editors and Affiliations

  1. College of Pharmacy, Dept. of Pharmaceutical Sciences, Oregon State University, SW Jefferson Street 1600, Corvallis, 97333, Oregon, USA

    Chrissa Kioussi

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Luder, H.U., Amstad-Jossi, M. (2012). Electron Microscopy. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_9

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  • DOI: https://doi.org/10.1007/978-1-61779-860-3_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-859-7

  • Online ISBN: 978-1-61779-860-3

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