Abstract
Short (or small) interfering RNAs (siRNAs) are double-stranded RNA molecules about 21–25 nucleotides long that have the capacity to disrupt the activity of genes on a posttranscriptional level. This sequence homology-driven gene silencing capacity has been utilized by researchers to selectively block the translation of mRNA to proteins in order to study specific gene functions and identify target molecules. Importantly, siRNAs have the potential to be used in treatment of disease. Here, we describe how the siRNA technology can be used to knock down genes in dental tissue-derived cells using integrin α11 knockdown as an example.
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Acknowledgments
This work was supported by University of Bergen, Bergen, Norway. The Research Council of Norway (183258/S10) and The Norwegian Cancer Society (536711) M.M. Barczyk is a recipient of grant from Helse Vest, Norway (project number: 911584).
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Barczyk, M.M., Gullberg, D., Bolstad, A.I. (2012). Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_6
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DOI: https://doi.org/10.1007/978-1-61779-860-3_6
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