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Lectin Array-Based Strategies for Identifying Metastasis-Associated Changes in Glycosylation

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Metastasis Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 878))

Abstract

Since 2005, lectin microarray technology has emerged as a relatively simple yet powerful technique for the comprehensive analysis of glycoprotein glycosylation. Lectin microarrays represent a new analytical method that can be used to explore the human glycome, a unique source of markers of diseases including cancer. The lectin microarray technology is a sensitive tool with the potential to allow high-throughput analysis of cancer-associated changes in glycosylation. This chapter describes the generation of a lectin-binding signature associated with metastatic primary breast tumours that have been resected, fixed, and embedded in paraffin. Procedures concerning sample and lectin microarray preparation are explained, alongside experimental considerations and approaches to data analysis.

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Author information

Authors and Affiliations

  1. Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK

    Simon Fry, Babak Afrough & Miriam Dwek

  2. Department of Molecular and Applied Biosciences, University of Westminster, London, UK

    Anthony Leathem

Authors
  1. Simon Fry
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  2. Babak Afrough
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  3. Anthony Leathem
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  4. Miriam Dwek
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Corresponding author

Correspondence to Miriam Dwek .

Editor information

Editors and Affiliations

  1. School of Life Sciences, Molecular and Applied Biosciences, University of Westminster, New Cavendish Street 115, London, W1W 6UW, United Kingdom

    Miriam Dwek

  2. School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, United Kingdom

    Susan A. Brooks

  3. Inst. Anatomie und Experimentelle, Morphologie, Universitätsklinikum Hamburg, Martinistr. 52, Hamburg, 20246, Germany

    Udo Schumacher

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Fry, S., Afrough, B., Leathem, A., Dwek, M. (2012). Lectin Array-Based Strategies for Identifying Metastasis-Associated Changes in Glycosylation. In: Dwek, M., Brooks, S., Schumacher, U. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 878. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-854-2_18

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  • DOI: https://doi.org/10.1007/978-1-61779-854-2_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-853-5

  • Online ISBN: 978-1-61779-854-2

  • eBook Packages: Springer Protocols

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