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Metastasis Research Protocols pp 185–199Cite as

Cell Separations by Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 878))

Abstract

Flow cytometry has become a standard method for separating individual subsets of cells from a heterogeneous population. Multilaser, multicolour cell sorters are increasingly common and have become more complex in recent years increasing the number of applications available. However, a cell sorting experiment is only as good as the input sample, and the preparation of this is extremely important. This chapter describes the methods used to prepare samples for flow cytometry and how they can be adapted and optimised according to cell type.

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Author information

Authors and Affiliations

  1. FACS Laboratory, London Research Institute, Cancer Research UK, London, UK

    Derek Davies

Authors
  1. Derek Davies
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Corresponding author

Correspondence to Derek Davies .

Editor information

Editors and Affiliations

  1. School of Life Sciences, Molecular and Applied Biosciences, University of Westminster, New Cavendish Street 115, London, W1W 6UW, United Kingdom

    Miriam Dwek

  2. School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, United Kingdom

    Susan A. Brooks

  3. Inst. Anatomie und Experimentelle, Morphologie, Universitätsklinikum Hamburg, Martinistr. 52, Hamburg, 20246, Germany

    Udo Schumacher

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Davies, D. (2012). Cell Separations by Flow Cytometry. In: Dwek, M., Brooks, S., Schumacher, U. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 878. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-854-2_12

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  • DOI: https://doi.org/10.1007/978-1-61779-854-2_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-853-5

  • Online ISBN: 978-1-61779-854-2

  • eBook Packages: Springer Protocols

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