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Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells

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Metastasis Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 878))

Abstract

The use of biosensors has become a standard method to characterize biomolecular interactions. Data obtained from biosensor studies are widely used to evaluate drug candidates, particularly in relation to their binding properties towards a selected target. The importance of measuring such interactions in a biologically relevant environment has become the new challenge for the biosensor technologies. In this chapter we describe a method for studying interactions between proteins and targets at the surface of intact cells using a quartz crystal microbalance (QCM) biosensor.

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References

  1. Curie J, Curie P (1880) Piezoelectric and allied phenomena in Rochelle salt. Comput Rend Acad Sci Paris 9:294–297

    Google Scholar 

  2. Sauerbrey G (1959) The use of quartz oscillators for weighing thin layers and for microweighing. Z Phys 155:206–222

    Article  CAS  Google Scholar 

  3. Koutsopoulos S, Unsworth LD, Nagai Y, Zhang S (2009) Controlled release of functional proteins through designer self-assembling peptide nanofiber hydrogel scaffold. Proc Natl Acad Sci USA 106:4623–4628

    Article  PubMed  CAS  Google Scholar 

  4. Pei Z, Anderson H, Aastrup T, Ramström O (2004) Study of real-time lectin-carbohydrate interactions on the surface of a quartz crystal microbalance. Biosens Bioelectron 21:60–66

    Article  PubMed  Google Scholar 

  5. Marx KA, Zhou T, Montrone A, McIntosh D, Braunhut SJ (2007) A comparative study of the cytoskeleton binding drugs nocodazole and taxol with a mammalian cell quartz crystal microbalance biosensor: different dynamic responses and energy dissipation effects. Anal Biochem 361:77–92

    Article  PubMed  CAS  Google Scholar 

  6. Myszka DG, Morton TA (1998) CLAMP: a biosensor kinetic data analysis program. Trends Biochem Sci 23:149–150

    Article  PubMed  CAS  Google Scholar 

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Saint-Guirons, J., Ingemarsson, B. (2012). Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells. In: Dwek, M., Brooks, S., Schumacher, U. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 878. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-854-2_11

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  • DOI: https://doi.org/10.1007/978-1-61779-854-2_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-853-5

  • Online ISBN: 978-1-61779-854-2

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