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Kidney Development pp 31–43Cite as

Live Imaging of the Developing Mouse Mesonephros

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 886))

Abstract

Embryonic development is a highly dynamic process involving complex tissue interactions and movements. Recent progress in cell labeling, image acquisition, and image processing technologies has brought the study of embryo morphogenesis to another level. It is now possible to visualize in real time the dynamic morphogenetic changes occurring in vivo and to reconstitute and quantify them in 4D rendering. However, extended live embryo imaging remains challenging in terms of embryo survival and minimization of phototoxicity. Here, we describe a procedure to image the developing mesonephros for up to 16 h in intact mouse embryos. This method can easily be adapted to the imaging of other structures at similar developmental stages.

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Acknowledgments

This work was supported by an operating grant from the Canadian Institutes for Health Research (CIHR; MOP-84470). M.B. holds a Canada Research Chair in Developmental Genetics of the Urogenital System.

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Authors and Affiliations

  1. Goodman Cancer Research Centre and Department of Biochemistry, McGill University, Montreal, QC, Canada

    David Grote & Michael Marcotte

  2. Goodman Cancer Research and Department of Biochemistry, McGill University, Montreal, QC, Canada

    Maxime Bouchard

Authors
  1. David Grote
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  2. Michael Marcotte
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  3. Maxime Bouchard
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Corresponding author

Correspondence to Maxime Bouchard .

Editor information

Editors and Affiliations

  1. Hammer Health Sciences Center Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA

    Odyssé Michos

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© 2012 Springer Science+Business Media, LLC

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Grote, D., Marcotte, M., Bouchard, M. (2012). Live Imaging of the Developing Mouse Mesonephros. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_4

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  • DOI: https://doi.org/10.1007/978-1-61779-851-1_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-850-4

  • Online ISBN: 978-1-61779-851-1

  • eBook Packages: Springer Protocols

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