Abstract
Obstructive nephropathy is the most important cause of renal failure in children. Unilateral ureteral obstruction (UUO) in the neonatal mouse provides a useful model to investigate the response of the developing kidney to urine flow obstruction. Creation of reversible variable partial UUO (compared to complete UUO) more closely approximates congenital lesions, and permits the study of recovery following release of the obstruction. Implementation of this technique requires the appropriate optical, surgical, and anesthetic equipment, as well as adaptations appropriate to the very small animals undergoing surgical procedures. Care of the pups must include minimizing trauma to delicate tissues, close monitoring of anesthesia and body temperature, and ensuring acceptance of the pups by the mother. It is important to document the severity and patency of the partial UUO by ureteral measurement and pelvic injection of India ink. Finally, removal of kidneys for histologic examination should be accomplished with gentle handling and processing.
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References
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Acknowledgments
We are grateful for the technical assistance and Fig. 4 provided courtesy of Michael Forbes, PhD. Funding has been provided by NIH grant RO1-DK803372 and a Career Enhancement Award from the University of Virginia Children’s Hospital.
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Thornhill, B.A., Chevalier, R.L. (2012). Variable Partial Unilateral Ureteral Obstruction and Its Release in the Neonatal and Adult Mouse. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_33
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DOI: https://doi.org/10.1007/978-1-61779-851-1_33
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