Abstract
In vitro culture of embryonic kidney rudiments has been utilized to study a variety of cellular processes and developmental mechanisms. Here, we describe two-dimensional (2D) culture of embryonic kidney rudiments on Transwell filters and three-dimensional (3D) cultures in collagen gels in detail, and 3D culture of isolated ureteric bud (UB) in Matrigel with BSN-conditioned media.
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References
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Acknowledgment
This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases grants (to S.K.N). The care and use of animals described in this investigation conform to the procedures of the laboratory’s Animal Protocol approved by the Animal Subjects Program and the IACUC of the University of California, San Diego. We thank Eran Rosines for creating the 3D projection of the branching ureteric buds (Fig. 2) from 2-photon images.
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Zhang, X., Bush, K.T., Nigam, S.K. (2012). In Vitro Culture of Embryonic Kidney Rudiments and Isolated Ureteric Buds. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_2
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DOI: https://doi.org/10.1007/978-1-61779-851-1_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-850-4
Online ISBN: 978-1-61779-851-1
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