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Engineered Tissues to Quantify Collective Cell Migration During Morphogenesis

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Kidney Development

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 886))

Abstract

Renal development is a complex process involving the dynamic interplay of over 25 different cell types. One distinct step in this process is the formation of the ureteric tree, which develops from the repeated branching of the ureteric bud. During branching of the ureteric bud, cells migrate collectively in unison to form the complex structure of the tree. Here, we present a microlithography-based 3D culture model in which multiple identical kidney epithelial tissues are used to quantify collective cell migration during the process of branching morphogenesis.

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References

  1. Costantini F, Kopan R (2010) Patterning a complex organ: branching morphogenesis and nephron segmentation in kidney development. Dev Cell 18:698–712

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Pohl M, Stuart RO, Sakurai H, Nigam SK (2000) Branching morphogenesis during kidney development. Annu Rev Physiol 62:595–620

    Article  CAS  PubMed  Google Scholar 

  3. Vasilyev A, Liu Y, Mudumana S et al (2009) Collective cell migration drives morphogenesis of the kidney nephron. PLoS Biol 7:e1000009

    Article  PubMed Central  Google Scholar 

  4. Sakurai H, Nigam SK (1998) In vitro branching tubulogenesis: Implications for developmental and cystic disorders, nephron number, renal repair, and nephron engineering. Kidney Int 54:14–26

    Article  CAS  PubMed  Google Scholar 

  5. Nelson CM, Inman JL, Bissell MJ (2008) Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression. Nat Protoc 3:674–678

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Nelson CM, VanDuijn MM, Inman JL, Fletcher DA, Bissell MJ (2006) Tissue geometry determines sites of mammary branching morphogenesis in organotypic cultures. Science 314:298–300

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Zaman M, Matsudaira P, Lauffenburger D (2007) Understanding effects of matrix protease and matrix organization on directional persistence and translational speed in three-dimensional cell migration. Ann Biomed Eng 35:91–100

    Article  PubMed  Google Scholar 

  8. Dickinson RB, Tranquillo RT (1993) Optimal estimation of cell movement indices from the statistical analysis of cell tracking data. AIChE J 39:1995–2010

    Article  Google Scholar 

  9. Dikeman DA, Rivera Rosado LA, Horn TA et al (2008) α4β1-Integrin regulates directionally persistent cell migration in response to shear flow stimulation. Am J Physiol Cell Physiol 295:C151–C159

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Mori H, Gjorevski N, Inman JL, Bissell MJ, Nelson CM (2009) Self-organization of engineered epithelial tubules by differential cellular motility. Proc Natl Acad Sci USA 106:14890–14895

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Rosello C, Ballet P, Planus E, Tracqui P (2004) Model driven quantification of individual and collective cell migration. Acta Biotheor 52:343–363

    Article  PubMed  Google Scholar 

  12. Parkhurst MR, Saltzman WM (1992) Quantification of human neutrophil motility in three-dimensional collagen gels. Effect of collagen concentration. Biophys J 61:306–315

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Zaman MH, Trapani LM, Sieminski AL et al (2006) Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell–matrix adhesion and proteolysis. Proc Natl Acad Sci USA 103:10889–10894

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Huang S, Brangwynne CP, Parker KK, Ingber DE (2005) Symmetry-breaking in mammalian cell cohort migration during tissue pattern formation: role of random-walk persistence. Cell Motil Cytoskeleton 61:201–213

    Article  CAS  PubMed  Google Scholar 

  15. ReinhartKing CA (2008) Endothelial cell adhesion and migration, In: Cheresh DA (ed) Methods in Enzymology. Academic Press, Oxford, pp 45–64

    Google Scholar 

  16. Arcasoy SM, Latoche J, Gondor M et al (1997) MUC1 and other sialoglycoconjugates inhibit adenovirus-mediated gene transfer to epithelial cells. Am J Respir Cell Mol Biol 17:422–435

    Article  CAS  PubMed  Google Scholar 

  17. O’Brien LE, Tang K, Kats ES et al (2004) ERK and MMPs sequentially regulate distinct stages of epithelial tubule development. Dev Cell 7:21–32

    Article  PubMed  Google Scholar 

  18. Shreiber DI, Tranquillo RT (2006) Three-dimensional, quantitative in vitro assays of wound healing behavior. In: Celis JE (ed) Cell biology: a laboratory handbook, 3rd edn. Academic Press, Oxford, p 2328

    Google Scholar 

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Acknowledgments

We thank Nikolce Gjorevski for assistance with the imaging and tracking software. This work was supported in part by grants from the NIH (CA128660 and GM083997), Susan G. Koman for the Cure (FAS0703855), the David and Lucile Packard Foundation, and the Alfred P. Sloan Foundation. C.M.N. holds a Career Award at Scientific Interface from the Burroughs Wellcome Fund.

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Authors and Affiliations

  1. Departments of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA

    Sriram Manivannan, Jason P. Gleghorn & Celeste M. Nelson

  2. Department of Molecular Biology, Princeton University, Princeton, NJ, USA

    Celeste M. Nelson

Authors
  1. Sriram Manivannan
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  2. Jason P. Gleghorn
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  3. Celeste M. Nelson
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Corresponding author

Correspondence to Celeste M. Nelson .

Editor information

Editors and Affiliations

  1. Hammer Health Sciences Center Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA

    Odyssé Michos

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Manivannan, S., Gleghorn, J.P., Nelson, C.M. (2012). Engineered Tissues to Quantify Collective Cell Migration During Morphogenesis. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_16

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  • DOI: https://doi.org/10.1007/978-1-61779-851-1_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-850-4

  • Online ISBN: 978-1-61779-851-1

  • eBook Packages: Springer Protocols

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