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In Ovo Eye Electroporation

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Retinal Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 884))

Abstract

Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells. Following entrance into the cytoplasm, the DNA is transported to the nucleus where it is transiently expressed. This powerful technique is useful for studies involving overexpression, misexpression, and knockdown of genes of interest at a variety of developmental timepoints.

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Acknowledgments

This work was supported by the American Health Assistance Foundation and NIH grants 1R01EY019525-01 and 1R15EY020816-01.

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Authors and Affiliations

  1. Department of Biology & Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA

    Teri L. Belecky-Adams, Scott R. Hudson & Sarika Tiwari

Authors
  1. Teri L. Belecky-Adams
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  2. Scott R. Hudson
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  3. Sarika Tiwari
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Corresponding author

Correspondence to Teri L. Belecky-Adams .

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Editors and Affiliations

  1. University of Alabama at Birmingham, University Blvd 444B, 1670, Birmingham, 35294-0019, Alabama, USA

    Shu-Zhen Wang

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Belecky-Adams, T.L., Hudson, S.R., Tiwari, S. (2012). In Ovo Eye Electroporation. In: Wang, SZ. (eds) Retinal Development. Methods in Molecular Biology, vol 884. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-848-1_5

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  • DOI: https://doi.org/10.1007/978-1-61779-848-1_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-847-4

  • Online ISBN: 978-1-61779-848-1

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