Abstract
Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).
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Acknowledgment
This work was supported by NIH grant EY004318 to PAR.
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Qin, Z., Raymond, P.A. (2012). Microarray-Based Gene Profiling Analysis of Müller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish. In: Wang, SZ. (eds) Retinal Development. Methods in Molecular Biology, vol 884. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-848-1_18
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DOI: https://doi.org/10.1007/978-1-61779-848-1_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-847-4
Online ISBN: 978-1-61779-848-1
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