Abstract
DNA sequencing is a powerful technique for identifying allelic variation within the natural killer cell immunoglobulin-like receptor genes. Because of the relatively large size of the KIR genes, each locus is amplified in two or more overlapping segments. Sanger sequencing of each gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.
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Hou, L., Chen, M., Steiner, N., Kariyawasam, K., Ng, J., Hurley, C.K. (2012). Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_25
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DOI: https://doi.org/10.1007/978-1-61779-842-9_25
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