Abstract
Polyacrylamide gel electrophoresis is a widely used technique for RNA analysis and purification. The polyacrylamide matrix is highly versatile for chemical derivitization, enabling facile exploitation of thio-mercury chemistry without the need of tedious manipulations and/or expensive coupling reagents, which often give low yields and side products. Here, we describe the use of [(N-acryloylamino)phenyl]mercuric chloride in three-layered polyacrylamide gels to detect, separate, quantify, and analyze sulfur-containing RNAs.
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Acknowledgments
This work was supported by National Science Foundation grant CHE-1057506 to Donald H. Burke.
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Biondi, E., Burke, D.H. (2012). Separating and Analyzing Sulfur-Containing RNAs with Organomercury Gels. In: Jin, H., Gassmann, W. (eds) RNA Abundance Analysis. Methods in Molecular Biology, vol 883. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-839-9_8
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DOI: https://doi.org/10.1007/978-1-61779-839-9_8
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