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Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs

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RNA Abundance Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 883))

Abstract

Northern analysis is a conventional but gold standard method for detection and quantification of gene expression changes. It not only detects the presence of a transcript but also indicates size and relative comparison of transcript abundance on a single membrane. In recent years, it has been aptly adapted to validate and study the size and expression of small noncoding RNAs. Here, we describe protocols employed in our laboratory for conventional northern analysis with total RNA/mRNA to study gene expression and validation of small noncoding RNAs using low molecular weight fraction of RNAs.

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Acknowledgments

Research work in the laboratories of S.K.-A. and M.A. is supported by grants from Department of Biotechnology (DBT), India and University of Delhi. R.P. and A.R.B. are thankful to Council of Scientific and Industrial Research (CSIR), India and DBT, India for the student fellowship, respectively.

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, India

    Ankur R. Bhardwaj & Manu Agarwal

  2. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India

    Ritu Pandey & Surekha Katiyar-Agarwal

Authors
  1. Ankur R. Bhardwaj
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  2. Ritu Pandey
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  3. Manu Agarwal
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  4. Surekha Katiyar-Agarwal
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Corresponding author

Correspondence to Surekha Katiyar-Agarwal .

Editor information

Editors and Affiliations

  1. , Dept. Plant Pathology & Microbiology, University of California, University Avenue 900, Riverside, 92521, USA

    Hailing Jin

  2. , Division of Plant Sciences, University of Missouri, Agriculture Building 1-31, Columbia, 65211, Missouri, USA

    Walter Gassmann

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Bhardwaj, A.R., Pandey, R., Agarwal, M., Katiyar-Agarwal, S. (2012). Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs. In: Jin, H., Gassmann, W. (eds) RNA Abundance Analysis. Methods in Molecular Biology, vol 883. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-839-9_2

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  • DOI: https://doi.org/10.1007/978-1-61779-839-9_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-838-2

  • Online ISBN: 978-1-61779-839-9

  • eBook Packages: Springer Protocols

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