Abstract
Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.
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References
de St F, Groth S, Webster RG, Datyner A (1963) Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim Biophys Acta 71:377–391
Wittig I, Braun HP, Schägger H (2006) Blue native PAGE. Nat Protoc 1:418–428
Neuhoff V, Arold N, Taube D et al (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255–262
Kang D, Gho YS, Suh M et al (2002) Highly sensitive and fast protein detection with coomassie brilliant blue in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bull Korean Chem Soc 11:1511–1512
Acknowledgments
Special thanks for excellent technical support to Christin Gräfe, Livia Schulze, and Barbara Uteß (Inst. Immunology, Dresden, Germany).
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Arndt, C., Koristka, S., Feldmann, A., Bartsch, H., Bachmann, M. (2012). Coomassie-Brilliant Blue Staining of Polyacrylamide Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_40
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DOI: https://doi.org/10.1007/978-1-61779-821-4_40
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-820-7
Online ISBN: 978-1-61779-821-4
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