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Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.

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References

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Acknowledgments

Special thanks for excellent technical support to Christin Gräfe, Livia Schulze, and Barbara Uteß (Inst. Immunology, Dresden, Germany).

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Correspondence to Michael Bachmann .

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© 2012 Springer Science+Business Media, LLC

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Arndt, C., Koristka, S., Feldmann, A., Bartsch, H., Bachmann, M. (2012). Coomassie-Brilliant Blue Staining of Polyacrylamide Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_40

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_40

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

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