Abstract
The efficient extraction of proteins of interest from cells and tissues is not always straightforward. In this process, the use of the optimal lysis buffer for protein solubilization should be considered. Here we demonstrate the use of a urea/thiourea lysis buffer, based on O’Farrell’s buffer, and compare its effectiveness for solubilization of proteins from smooth muscle with the often utilized RIPA lysis buffer.
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Acknowledgments
This work was supported by a Natural Sciences and Engineering Research Council Discovery Grant (#250218) to DJM. MP was a holder of an Alexander Graham Bell NSERC postgraduate fellowship.
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Peach, M., Marsh, N., MacPhee, D.J. (2012). Protein Solubilization: Attend to the Choice of Lysis Buffer. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_4
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DOI: https://doi.org/10.1007/978-1-61779-821-4_4
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