Abstract
The use of electrophoretic methodologies for use in qualitative and preparative purposes are commonly used in biological research and have been well established as an integral analytical tool for a long time in most research laboratories. Listed here are some of the more specialized innovations that have been developed in recent times for special purposes of study. These include micropreparative isoelectric focusing in liquid suspension, accelerated protease digestion by SDS-PAGE, two-dimensional SDS-PAGE for membrane protein resolution, carbon nanotube-modified page for resolution of complement C3, electrophoretic resolution of ultra-acidic proteomes in acidic media, and two-dimensional immunoelectrophoresis of pre-beta/alpha lipoprotein A-I in agarose. All these methods are briefly reviewed in this chapter.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Egatz-Gomez A, Thormann W (2011) Micropreparative isoelectric focusing protein separation in a suspended drop. Electrophoresis 32:1433–1437
Bornemann S, Rietschel B, Baltruschat S et al (2010) A novel polyacrylamide gel system for proteomic use offering controllable pore expansion by crosslinker cleavage. Electrophoresis 31:585–592
Williams TI, Combs JC, Thakur AP et al (2006) A novel Bicine running buffer system for doubled sodium dodecyl sulfate - polyacrylamide gel electrophoresis of membrane proteins. Electrophoresis 27:2984–2995
Jiang F, Wang Y, Hu X et al (2010) Carbon nanotubes-assisted polyacrylamide gel electrophoresis for enhanced separation of human serum proteins and application in liverish diagnosis. J Sep Sci 33:3393–3399
Hempel K, Rosen R, Becher D et al (2009) Analysis of ultra acidic proteins by the use of anodic acidic gels. Anal Biochem 385:208–214
Laurel CB (1965) Antigen-antibody crossed electrophoresis. Anal Biochem 10:358
Weeke B (1973) Crossed immunoelectrophoresis. In: Axelsen NH, KrÓ©ll J, Weeke B (eds) Quantitative immunoelectrophoresis. UniverÂsitetsforlaget, Oslo
Fielding PE, Miida T, Fielding CJ (1991) Metabolism of low-density lipoprotein free cholesterol by human plasma lecithin-cholesterol acyltransferase. Biochemistry 30:8551–8557
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Fesmire, J.D. (2012). A Brief Review of Other Notable Electrophoretic Methods. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_38
Download citation
DOI: https://doi.org/10.1007/978-1-61779-821-4_38
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-820-7
Online ISBN: 978-1-61779-821-4
eBook Packages: Springer Protocols