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Protein Identification on Archived 2-D Gels

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

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Abstract

Because of the availability of genome information combined with proteomics techniques, it is possible to determine the identity of a protein which had been isolated many years ago on a two-dimensional gel electrophoresis and stored in a dry state as a data archive. The protocol described in this chapter will assist researchers who want to know the identity of a protein separated decades ago when no techniques were available to determine the identity of the protein.

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References

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Correspondence to Hiroyuki Matsumoto .

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Matsumoto, H., Takemori, N., Komori, N. (2012). Protein Identification on Archived 2-D Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_25

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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