Abstract
Because of the availability of genome information combined with proteomics techniques, it is possible to determine the identity of a protein which had been isolated many years ago on a two-dimensional gel electrophoresis and stored in a dry state as a data archive. The protocol described in this chapter will assist researchers who want to know the identity of a protein separated decades ago when no techniques were available to determine the identity of the protein.
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References
Matsumoto H, Komori N (2000) Ocular proteomics: cataloging photoreceptor proteins by two-dimensional gel electrophoresis and mass spectrometry. Methods Enzymol 316:492–511
Matsumoto H, Kurono S, Matsumoto M et al (2005) Mass spectrometry of biomolecules in proteomics. In: Meyers RA (ed) Encyclopedia of molecular and cell biology and molecular medicine. Wiley-VCH Verlag GmbH, Germany, pp 557–585
Matsumoto H, Komori N (1999) Protein identification on two-dimensional gels archived nearly two decades ago by in-gel digestion and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Anal Biochem 270:176–179
Rosenfeld J, Capdevielle J, Guillemot JC et al (1992) In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal Biochem 203: 173–179
Matsumoto H, Kurien BT, Takagi Y et al (1994) Phosrestin I undergoes the earliest light-induced phosphorylation by a calcium/calmodulin-dependent protein kinase in Drosophila photoreceptors. Neuron 12: 997–1010
Kinumi T, Tobin SL, Matsumoto H et al (1997) The phosphorylation site and desmethionyl N-terminus of drosophila phosrestin I in vivo determined by mass spectrometric analysis of proteins separated on two-dimensional gel electrophoresis. Eur Mass Spectrom 3:367–378
Takemori N, Komori N, Matsumoto H (2006) Highly sensitive multistage mass spectrometry enables small scale analysis of protein glycosylation from two-dimensional polyacrylamide gel. Electrophoresis 27:1394–1406
Takemori N, Komori N, Thompson J Jr et al (2007) Novel eye-specific calmodulin methylation characterized by protein mapping in Drosophila melanogaster. Proteomics 7:2651–2658
Takemori N, Komori N, Matsumoto H (2009) MS analysis of protein glycosylation, Chapter 150. In: Walker JM (ed) The protein protocols handbook, 3rd edn. Humana, New York, pp 1381–1388
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Matsumoto, H., Takemori, N., Komori, N. (2012). Protein Identification on Archived 2-D Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_25
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DOI: https://doi.org/10.1007/978-1-61779-821-4_25
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-820-7
Online ISBN: 978-1-61779-821-4
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