Abstract
An overview of the methods for assessing cell viability is presented. Different protocols of the most commonly used assays are described in detail so that the readers may be able to determine which assay is suitable for their own projects in plant biotechnology.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Vanková R, Kuncová G, Opatmá J et al (2001) Two-dimensional fluorescence spectroscopy – a new tool for the determination of plant cell viability. Plant Cell Rep 20:41–47
Wei N, You J, Friehs K et al (2007) In situ dark field microscopy for on-line monitoring of yeast cultures. Biotechnol Lett 29:373–378
Palta JP, Levitt J, Stadeimann EJ (1978) Plant viability assay. Cryobiology 15:249–255
Pegg DE (1989) Viability assays for preserved cells, tissues and organs. Cryobiology 26: 212–231
Smith BA, Reider ML, Fletcher JS (1982) Relationship between vital staining and subculture growth during the senescence of plant tissue culture. Plant Physiol 70:1228–1230
Baker CJ, Mock MM (1994) An improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue. Plant Cell Tissue Organ Cult 39:7–12
Huang CN, Cornejo MJ, Bush DS et al (1986) Estimating viability of plant protoplasts using double and single staining. Protoplasma 135:80–87
Hou BH, Lin CG (1996) Rapid optimization of electroporation conditions for soybean and tomato suspension cultured cells. Plant Physiol 111(Suppl 2):166
Cripen RW, Perrier JL (1974) The use of neutral red and Evans blue for live-dead determinations of marine plankton. Stain Technol 49: 97–104
Wildholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47:189–194
Towill LE, Mazur P (1975) Studies on the reduction of 2,3,5- triphenyltetrazolium chloride as viability assay for plant tissue cultures. Can J Bot 53:1097–1102
Moreira-Quiroz J, Valencia-Delgado V, Chávez-Burbano P (2009) Implementación de un algoritmo para la detección y conteo de células en imágenes microscópicas. http://www.dspace.espol.edu.ec/handle/123456789/8375. Accessed 30 Nov 2010
Steward N, Martin R, Engasser JM (1999) A new methodology for plant cell viability assessment using intracellular esterase activity. Plant Cell Rep 19:171–176
Carvell JP, Cannizzaro C (2007) On-line biomass monitoring in cell culture with scanning radio-frequency impedance spectroscopy. In: Smith R (ed) Cell technology for cell products. Springer, New York
Wei N, Flaschel E, Friehs K et al (2008) A machine vision system for non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification. BMC Bioinformatics 9:449–463
Kranner I, Kastberger G, Hartbauer M et al (2010) Noninvasive diagnosis of seed viability using infrared thermography. Proc Natl Acad Sci USA 107:3912–3917
Van Benthem R, de Grave W (2009) Turbidity sensor for bacterial growth measurements in spaceflight and simulated micro-gravity. Microgravity Sci Technol 21:349–356
Kodama M, Yoshida T, Otani H et al (1991) Effect of AL-toxin on viability of cultured tomato cells determined by the MTT colorimetric assay. In: Patil S (ed) Molecular strategies of pathogens and host plants. Springer, Berlin
Vera-Estrella R, Blumwald E, Higgins VJ (1992) Effects of specific elicitors of Cladosporium fulvum on tomato suspension cells. Evidence for the involvement of active oxygen species. Plant Physiol 99:1208–1215
Sánchez LM, Doke N, Kawakita K (1993) Elicitor induced chemiluminescence in cell suspension cultures of tomato, sweet pepper and tobacco plants and its inhibition by suppressors from Phytophthora spp. Plant Sci 88:141–148
Escobedo RM, Miranda-Ham ML (2003) Analysis of elicitor-induced cell viability changes in Lycopersicon esculentum Mill. suspension culture by different methods. In Vitro Cell Dev Biol Plant 39:236–239
Castro-Concha LA, Escobedo RM, Miranda-Ham ML (2006) Measurement of cell viability in in vitro cultures. In: Loyola-Vargas VM, Vázquez-Flota F (eds) Plant cell culture protocols. Humana, Totowa
Rivera-Madrid R, Escobedo-GM RM, Balam-Galera E et al (2006) Preliminary studies toward genetic improvement of annatto (Bixa orellana L). Sci Hortic 109:165–172
Dolcet-Sanjuan R, Claveria E, Huerta A (1997) Androgenesis in Capsicum annuum L.: effects of carbohydrate and carbon dioxide enrichment. J Am Soc Hort Sci 122:468–475
Rodríguez-Riano T, Dafni A (2000) A new procedure to asses pollen viability. Sex Plant Reprod 12:241–244
Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Castro-Concha, L.A., Escobedo, R.M., de Lourdes Miranda-Ham, M. (2012). Measurement of Cell Viability. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-61779-818-4_5
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-817-7
Online ISBN: 978-1-61779-818-4
eBook Packages: Springer Protocols