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Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 877))

Abstract

In vitro culture is one of the most studied techniques, and it is used to study many developmental processes, especially in forestry species, because of growth timing and easy manipulation. Epigenetics has been shown as an important influence on many research analyses such as cancer in mammals and developmental processes in plants such as flowering, but regarding in vitro culture, techniques to study DNA methylation or chromatin modifications were mainly limited to identify somaclonal variation of the micropropagated material. Because in vitro culture is not only a way to generate plant material but also a bunch of differentially induced developmental processes, an approach of techniques and some research carried out to study the different changes regarding DNA methylation and chromatin and translational modifications that take place during these processes is reviewed.

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Authors and Affiliations

  1. Área de Fisiología Vegetal, Depto. BOS, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain

    José L. Rodríguez, Jesús Pascual, Marcos Viejo, Norma Yague Yrei, Marta Pérez, Roberto Rodríguez Fernández & María J. Cañal

  2. Instituto de Biotecnología de Asturias (asociado al CSIC), Oviedo, Spain

    José L. Rodríguez, Jesús Pascual, Marcos Viejo, Norma Yague Yrei, Marta Pérez, Roberto Rodríguez Fernández & María J. Cañal

  3. Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, Vienna, Austria

    Luis Valledor

  4. Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria

    Mónica Meijón

  5. Facultad de Ciencias Forestales, Universidad de Concepción, Concepción, Chile

    Rodrigo Hasbún

  6. Department of Biology, WSC 339/341, The University of Western Ontario, London, Ontario, Canada

    María E. Santamaría

  7. Department of Endocrinology and Nutrition Service, Hospital Universitario Central de Asturias, Oviedo, Spain

    Mario Fernández Fraga

  8. Cancer Epigenetics and Biology Program, IDIBELL, Barcelona, Spain

    María Berdasco

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  1. José L. Rodríguez
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  2. Jesús Pascual
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  3. Marcos Viejo
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  4. Luis Valledor
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  5. Mónica Meijón
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  6. Rodrigo Hasbún
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  7. Norma Yague Yrei
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  8. María E. Santamaría
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  10. Mario Fernández Fraga
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  12. Roberto Rodríguez Fernández
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  13. María J. Cañal
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Corresponding author

Correspondence to José L. Rodríguez .

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Editors and Affiliations

  1. , Unidad de Bioquímica y Biología Molecula, Centro de Investigación Científica de Yu, Apartado Postal 87, Cordemex, Mérida, Mexico

    Víctor M. Loyola-Vargas

  2. , Departamento de Ingeniería Genética de P, Ctr Investigación, Estudios Avanzados In, Apartado Postal 629, Irapuato, 36821, Mexico

    Neftalí Ochoa-Alejo

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Rodríguez, J.L. et al. (2012). Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_25

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  • DOI: https://doi.org/10.1007/978-1-61779-818-4_25

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