Abstract
microRNAs (miRNAs), a class of small, endogenous, noncoding RNAs, are uncovered to play greatly expanded roles in a variety of plant developmental processes by gene silencing through inhibiting translation or promoting the degradation of target mRNAs. In virtue of their ability to inactivate either specific genes or entire gene families, artificial miRNAs function as dominant suppressors of gene activity when brought into a plant. Moreover, artificial target mimics are applied for the reduction of specific miRNA activity. Consequently, miRNA-based manipulations have emerged as promising new approaches for the improvement of crop plants. This action includes the development of breeding strategies and the genetic modification of agronomic traits. Herein, we describe the current miRNA-based plant engineering approaches, and their advantages and challenges are also stated.
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Brown ME, Funk CC (2008) Climate. Food security under climate change. Science 319: 580–581
Liu Q, Chen YQ (2010) A new mechanism in plant engineering: the potential roles of microRNAs in molecular breeding for crop improvement. Biotechnol Adv 28:301–307
Jones-Rhoades MW, Bartel DP, Bartel B (2006) MicroRNAs and their regulatory roles in plants. Ann Rev Plant Biol 57:19–53
Chuck G, Candela H, Hake S (2009) Big impacts by small RNAs in plant development. Curr Opin Plant Biol 12:81–86
Liu Q, Chen YQ (2009) Insights into the mechanism of plant development: interactions of miRNAs pathway with phytohormone response. Biochem Biophys Res Commun 384:1–5
Chen J, Li WX, Xie DX et al (2004) Viral virulence protein suppresses RNA silencing-mediated defense but upregulates the role of MicroRNA in host gene expression. Plant Cell 16:1302–1313
Sunkar R, Kapoor A, Zhu JK (2006) Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance. Plant Cell 18:2415
Franco-Zorrilla JM, Valli A, Todesco M et al (2007) Target mimicry provides a new mechanism for regulation of microRNA activity. Nat Genet 39:1033–1037
Todesco M, Rubio-Somoza I, Paz-Ares J, Weigel D (2010) A collection of target mimics for comprehensive analysis of microRNA function in Arabidopsis thaliana. PLoS Genet 6:e1001031
Duan CG, Wang CH, Fang RX, Guo HS (2008) Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. J Virol 82:11084–11095
Schwab R, Ossowski S, Riester M et al (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18:1121–1133
Qu J, Ye J, Fang R (2007) Artificial microRNA-mediated virus resistance in plants. J Virol 81:6690–6699
Khraiwesh B, Ossowski S, Weigel D et al (2008) Specific gene silencing by artificial MicroRNAs in Physcomitrella patens: an alternative to targeted gene knockouts. Plant Physiol 148:684–693
Tretter EM, Alvarez JP, Eshed Y, Bowman JL (2008) Activity range of Arabidopsis small RNAs derived from different biogenesis pathways. Plant Physiol 147:58–62
Niu QW, Lin SS, Reyes JL et al (2007) Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat Biotechnol 25:254
Warthmann N, Chen H, Ossowski S et al (2008) Highly specific gene silencing by artificial miRNAs in rice. PLoS One 3:e1829
Zhao T, Wang W, Bai X, Qi YJ (2009) Gene silencing by artificial microRNAs in Chlamydomonas. Plant J 58:157–164
Shi R, Yang C, Lu S et al (2010) Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa. Planta 232: 1281–1288
Fujii H, Chiou TJ, Lin SI et al (2005) A miRNA involved in phosphate-starvation response in Arabidopsis. Curr Biol 15:2038–2043
Ebert MS, NeilsonM JR, Sharp PA (2007) MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods 4:721–726
Ossowski S, Schwab R, Weigel D (2008) Gene silencing in plants using artificial microRNAs and other small RNAs. Plant J 53: 674–690
Kim J, Somers DE (2010) Rapid assessment of gene function in the circadian clock using artificial microRNA in Arabidopsis thaliana mesophyll protoplasts. Plant Physiol 154: 611–621
Park W, Zhai J, Lee JY (2009) Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CA1 genes. Plant Cell Rep 28:469–480
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Liu, Q., Chen, YQ. (2012). The Potential Roles of microRNAs in Molecular Breeding. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_23
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DOI: https://doi.org/10.1007/978-1-61779-818-4_23
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Publisher Name: Humana Press, Totowa, NJ
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