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Epigenetics, the Role of DNA Methylation in Tree Development

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 877))

Abstract

During development of multicellular organisms, cells become differentiated by modulating different programs of gene expression. Cells have their own epigenetic signature which reflects genotype, developmental history, and environmental influences, and it is ultimately reflected in the phenotype of the cells and the organism. However, in normal development or disease situations, such as adaptation to climate change or during in vitro culture, some cells undergo major epigenetic reprogramming involving the removal of epigenetic marks in the nuclei followed by the establishment of a different new set of marks. Compared with animal cells, biotech-mediated achievements are reduced in plants despite the presence of cell polypotency. In forestry, any sustainable developments using biotech tools remain restricted to the lab, without progressing to the field for application. Such barriers in the translation between development and implementation need to be addressed by organizations that have the power to integrate these two fields. However, a lack of understanding of gene regulation is also to blame for this barrier. In recent years, great progress has been made in unraveling the control of gene expression. These advances are discussed in this chapter, including the possibility of applying this knowledge in forestry practice.

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Acknowledgments

Scientific progress in aging, phase change, reinvigoration, and markers for quality was made with financial support from EU Projects FAIR3-CT96-1445, INCO 10063, and MCT-AGL2000-2126, AGL 2004-00810/FOR, AGL2007-62907/FOR Spanish National Projects. The Spanish M.E.C.D. supported fellowships of all young researchers.

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Authors and Affiliations

  1. Área de Fisiología Vegetal, Depto. BOS, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain

    Marcos Viejo, José L. Rodríguez, Marta Pérez, Jesús Pascual, María J. Cañal & Roberto Rodríguez Fernández

  2. Instituto de Biotecnología de Asturias (asociado al CSIC), Oviedo, Spain

    Marcos Viejo, José L. Rodríguez, Marta Pérez, Jesús Pascual, María J. Cañal & Roberto Rodríguez Fernández

  3. Department of Biology, WSC 339/341, The University of Western Ontario, London, Ontario, Canada

    María E. Santamaría

  4. Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, Vienna, Austria

    Luis Valledor

  5. Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria

    Mónica Meijón

  6. Facultad de Ciencias Forestales, Universidad de Concepción, Concepción, Chile

    Rodrigo Hasbún

  7. Department of Endocrinology and Nutrition Service, Hospital Universitario Central de Asturias, Oviedo, Spain

    Mario Fernández Fraga

  8. Cancer Epigenetics and Biology Program, IDIBELL, Barcelona, Spain

    María Berdasco

  9. Seed Conservation Department, Royal Botanic Gardens Kew, Wakehurst Place, West Sussex, UK

    Peter E. Toorop

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  1. Marcos Viejo
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  2. María E. Santamaría
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  6. Marta Pérez
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  7. Jesús Pascual
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  8. Rodrigo Hasbún
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  9. Mario Fernández Fraga
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  10. María Berdasco
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  11. Peter E. Toorop
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  12. María J. Cañal
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  13. Roberto Rodríguez Fernández
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Corresponding author

Correspondence to Marcos Viejo .

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Editors and Affiliations

  1. , Unidad de Bioquímica y Biología Molecula, Centro de Investigación Científica de Yu, Apartado Postal 87, Cordemex, Mérida, Mexico

    Víctor M. Loyola-Vargas

  2. , Departamento de Ingeniería Genética de P, Ctr Investigación, Estudios Avanzados In, Apartado Postal 629, Irapuato, 36821, Mexico

    Neftalí Ochoa-Alejo

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Viejo, M. et al. (2012). Epigenetics, the Role of DNA Methylation in Tree Development. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_22

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  • DOI: https://doi.org/10.1007/978-1-61779-818-4_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-817-7

  • Online ISBN: 978-1-61779-818-4

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