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Human Salivary Gland Stem Cells: Isolation, Propagation, and Characterization

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Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Stem cells are of outstanding interest for a variety of applications in regenerative medicine. The identification and characterization of novel tissue sources in order to reduce donor site morbidity and to provide specific cells in clinically applicable numbers have led to the detection of stem cells in almost all adult tissues. Salivary glands are of specific interest to our lab, as these tissues are easily accessible for the head and neck surgeon with low donor site morbidity. On the other hand, they possess an endocrine and exocrine function and thus play a very specific role in the human body. Stem cell identity however can only be demonstrated using a combination of different methods in vitro, as there is not a single marker or feature allowing for definite identification of such cells. In this chapter, we provide a comprehensive summary of our experimental methods for the isolation and characterization of human salivary gland stem cells in vitro.

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Acknowledgment

The authors thank M. Jerg and K. Urlbauer for the excellent technical assistance. This work was supported by a grant from the German-Israeli Foundation.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Ulm University Medical Center, Ulm, Germany

    Silke Schwarz & Nicole Rotter

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  1. Silke Schwarz
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  2. Nicole Rotter
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Correspondence to Nicole Rotter .

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  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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Schwarz, S., Rotter, N. (2012). Human Salivary Gland Stem Cells: Isolation, Propagation, and Characterization. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_25

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

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