Skip to main content
SpringerLink
Log in

A Unique FACS Method to Isolate Stem Cells in Planarian

  • Protocol
  • First Online:
Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Fluorescence-activated cell sorting (FACS) is a useful method for stem cell biology, which enables us to isolate the living stem cells of interest from mixture of a variety of cells. In general, the target cells for FACS need to be labeled for various cell surface markers. However, in non-model organisms, we usually do not have specific labels for such cell surface markers. Here, we describe a method for isolating stem cells from non-model organisms, such as planarians, based on physiological and morphological properties of the stem cells. This method may also be applicable to other non-model animals.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Spangrude GJ, Heimfeld S, Weissman IL (1988) Purification and characterization of mouse hematopoietic stem cells. Science 241:58–62

    Article  PubMed  CAS  Google Scholar 

  2. Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC (1996) Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 183:1797–1806

    Article  PubMed  CAS  Google Scholar 

  3. Wolff E (1962) Recent research on the regeneration of planaria. In: Rudnick D (ed) Regeneration. 20th Growth Symposium. Ronald Press, New York, pp 53–84

    Google Scholar 

  4. Agata K, Watanabe K (1999) Molecular and cellular aspects of planarian regeneration. Semin Cell Dev Biol 10:377–383

    Article  PubMed  CAS  Google Scholar 

  5. Baguñà J (1981) Planarian neoblasts. Nature 290:14–15

    Article  Google Scholar 

  6. Hayashi T, Asami M, Higuchi S, Shibata N, Agata K (2006) Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting. Dev Growth Differ 48:371–380

    Article  PubMed  Google Scholar 

  7. Hayashi T, Shibata N, Okumura R, Kudome K, Nishimura O, Tarui H, Agata K (2010) Single-cell gene profiling of planarian stem cells utilizing FACS and its “index sorting” function for stem cell research. Dev Growth Differ 52:131–144

    Article  PubMed  CAS  Google Scholar 

  8. Higuchi S, Hayashi T, Hori I, Shibata N, Sakamoto H, Agata K (2007) Characterization and categorization of fluorescence activated cell sorted planarian stem cells by ultrastructural analysis. Dev Growth Differ 49: 571–581

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We thank all our laboratory members for helpful discussions and for technical guidance and Dr. Elizabeth Nakajima for critical reading of the manuscript.

Author information

Authors and Affiliations

  1. Genome Resource and Analysis Unit, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo, Japan

    Tetsutaro Hayashi

  2. Department of Biophysics, Kyoto University, Sakyo-ku, Kyoto, Japan

    Kiyokazu Agata

Authors
  1. Tetsutaro Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kiyokazu Agata
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kiyokazu Agata .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Hayashi, T., Agata, K. (2012). A Unique FACS Method to Isolate Stem Cells in Planarian. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-815-3_2

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation