Abstract
Isolation of prostate epithelial cells with stem/progenitor characteristics may enable further evaluation of the hierarchy of prostate glandular development and malignant transformation. Prostate epithelial cells capable of sphere formation in semisolid cultures possess stem/progenitor cell characteristics. This is demonstrated by self-renewal (via indefinite passaging) and in vivo differentiation into prostate tubules with discreet basal and luminal layers. Here, we describe a method for isolating prostate stem/progenitor cells from human tissues via in vitro prostasphere formation. Prostate tissue regeneration using human prostaspheres is also described, enabling the differentiation potential of sphere-forming cells to be observed.
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Acknowledgment
This work was supported by the Jean Perkins Foundation, the Prostate Cancer Foundation, and the Department of Defense. FACS sorting parameters were provided by the Janis V. Giorgi Flow Cytometry Laboratory in the Jonsson Cancer Comprehensive Cancer Center at UCLA.
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Guo, C., Zhang, B., Garraway, I.P. (2012). Isolation and Characterization of Human Prostate Stem/Progenitor Cells. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_18
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DOI: https://doi.org/10.1007/978-1-61779-815-3_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-814-6
Online ISBN: 978-1-61779-815-3
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