Abstract
Functional redundancy widely exists in genes encoding receptors and signaling components of plant hormones, particularly the stress hormone abscisic acid (ABA). The redundancy hinders the use of conventional genetic approach to dissecting molecular mechanisms for ABA signal perception and transduction. Chemical genetics approach, in which bioactive small molecules are used to perturb the function of gene products encoded by functionally redundant genes, provides an excellent alternative strategy to investigate ABA signaling. This approach led to the discovery of ABA receptor family, PYR/PYL/RCAR (Zhao et al., Nat Chem Biol 3:716–721, 2007; Park et al., Science 324:1068–1071, 2009). A forward chemical genetics screen uncovered an ABA agonist, pyrabactin, which provides a critical small-molecule probe to the identification of the ABA receptor family and the dissection of its downstream signaling.
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References
Sirichandra C, Wasilewska A, Vlad F, Valon C, Leung J (2009) The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action. J Exp Bot 60:1439–1463
Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu J-K (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539
Ton J, Flors V, Mauch-Mani B (2009) The multifaceted role of ABA in disease resistance. Trends Plant Sci 14:310–317
Weitbrecht K, Muller K, Leubner-Metzger G (2011) First off the mark: early seed germination. J Exp Bot 62(10):3289–3309
Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol 61:651–679
Toth R, van der Hoorn RAL (2010) Emerging principles in plant chemical genetics. Trends Plant Sci 15:81–88
Sebolt-Leopold JS, Dudley DT, Herrera R, Van Becelaere K, Wiland A, Gowan RC, Tecle H, Barrett SD, Bridges A, Przybranowski S, Leopold WR, Saltiel AR (1999) Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nat Med 5:810–816
Stockwell BR (2000) Chemical genetics: ligand-based discovery of gene function. Nat Rev Genet 1:116–125
Zhao Y, Chow TF, Puckrin RS, Alfred SE, Korir AK, Larive CK, Cutler SR (2007) Chemical genetic interrogation of natural variation uncovers a molecule that is glycoactivated. Nat Chem Biol 3:716–721
Tsuchiya Y, Vidaurre D, Toh S, Hanada A, Nambara E, Kamiya Y, Yamaguchi S, McCourt P (2010) A small-molecule screen identifies new functions for the plant hormone strigolactone. Nat Chem Biol 6:741–749
Schreiber K, Ckurshumova W, Peek J, Desveaux D (2008) A high-throughput chemical screen for resistance to Pseudomonas syringae in Arabidopsis. Plant J 54:522–531
Smukste I, Stockwell BR (2005) Advances in chemical genetics. Annu Rev Genom Hum G 6:261–286
Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu JK, Schroeder JI, Volkman BF, Cutler SR (2009) Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 324(5930):1068–1071
Yuji Suzuki TK, Koyama H (2004) RNA isolation from siliques, dry seeds, and other tissues of Arabidopsis thaliana. Biotechniques 37:542–544
Acknowledgments
The author feels very grateful to have chance to join some very exciting and pioneer works in plant chemical genetics research when studying in Dr. Sean Cutler lab. The author would like to thank Ms. Peiling Zhang from Zhao lab for her kind helping to prepare the manuscript.
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Zhao, Y. (2011). A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination. In: Wang, ZY., Yang, Z. (eds) Plant Signalling Networks. Methods in Molecular Biology, vol 876. Humana Press. https://doi.org/10.1007/978-1-61779-809-2_8
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DOI: https://doi.org/10.1007/978-1-61779-809-2_8
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