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Spectroscopic Methods of Analysis pp 57–65Cite as

Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence Technique

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 875))

Abstract

Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug’s association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

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Authors and Affiliations

  1. Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland

    Wieslaw I. Gruszecki, Rafal Luchowski & Piotr Wasko

  2. Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA

    Zygmunt Gryczynski & Ignacy Gryczynski

Authors
  1. Wieslaw I. Gruszecki
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  2. Rafal Luchowski
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  3. Piotr Wasko
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  4. Zygmunt Gryczynski
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  5. Ignacy Gryczynski
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Corresponding author

Correspondence to Wieslaw I. Gruszecki .

Editor information

Editors and Affiliations

  1. Dept. Biochemistry & Molecular Biology, University of Texas Medical Branch, University Blvd. 301, Galveston, 77555-1053, Texas, USA

    Wlodek M. Bujalowski

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Gruszecki, W.I., Luchowski, R., Wasko, P., Gryczynski, Z., Gryczynski, I. (2012). Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence Technique. In: Bujalowski, W. (eds) Spectroscopic Methods of Analysis. Methods in Molecular Biology, vol 875. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-806-1_3

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  • DOI: https://doi.org/10.1007/978-1-61779-806-1_3

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-805-4

  • Online ISBN: 978-1-61779-806-1

  • eBook Packages: Springer Protocols

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