Abstract
The GPCR-coupled sphingosine-1-phosphate (S1P) receptors regulate a number of important cell functions, including proliferation, migration, and adhesion. Since these processes require dynamic regulation of the actin cytoskeleton, the ability to monitor S1P-dependent activation of the Rho family GTPases is critical for our understanding of S1P signaling. Herein, we provide methods for the GST pull-down-based assay used to measure Rho, Rac, and Cdc42 activity in cultured cells treated with S1P.
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Medlin, M.D., Taylor, J.M., Mack, C.P. (2012). Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 874. Humana Press. https://doi.org/10.1007/978-1-61779-800-9_8
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DOI: https://doi.org/10.1007/978-1-61779-800-9_8
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