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Sphingosine-1-Phosphate pp 177–192Cite as

Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 874))

Abstract

Recent studies show that bioactive lipids are important regulators for stem cell survival and differentiation. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically on embryonic stem (ES) cell differentiation. We show here simple methods to analyze sphingolipids in differentiating ES cells and to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

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Acknowledgements

This study was in part supported by the NIH grants R01AG034389 and R01NS046835, a GRAVentureLab grant, and a March of Dimes grant 6-FY08-322.

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Authors and Affiliations

  1. Program in Developmental Neurobiology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Georgia Health Sciences University, Augusta, GA, USA

    Erhard Bieberich

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  1. Erhard Bieberich
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Correspondence to Erhard Bieberich .

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Editors and Affiliations

  1. University of Melbourne, Parkville, Victoria, Australia

    Alice Pébay

  2. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Bieberich, E. (2012). Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 874. Humana Press. https://doi.org/10.1007/978-1-61779-800-9_14

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  • DOI: https://doi.org/10.1007/978-1-61779-800-9_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-799-6

  • Online ISBN: 978-1-61779-800-9

  • eBook Packages: Springer Protocols

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