Abstract
Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules (3H, 32P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.
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Acknowledgments
This work was supported by fundings from the Institut National du Cancer (INCa), La Ligue Nationale Contre le Cancer (Equipe labellisée), La Ligue Contre le Cancer (Comité 31), Association pour la Recherche sur le Cancer (ARC), Association pour la Recherche sur les Tumeurs de la Prostate (ARTP), Université Paul Sabatier de Toulouse.
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Brizuela, L., Cuvillier, O. (2012). Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 874. Humana Press. https://doi.org/10.1007/978-1-61779-800-9_1
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DOI: https://doi.org/10.1007/978-1-61779-800-9_1
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Publisher Name: Humana Press
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